Simultaneous surface display and secretion of antibodies Simultaneous Surface Display and Secretion of Proteins from Mammalian Cells Facilitates Efficient in vitro Selection and Maturation of Antibodies
نویسندگان
چکیده
Background: Simultaneous cell surface display and secretion is desirable for protein evolution and selection. Results: Alternative splicing enables simultaneous cell surface display and secretion of the same protein or an alternate form to facilitate screening. Conclusion: Analysis of secreted protein complements cell surface display for evolution of protein function. Significance: This technology can enable rapid evolution and characterization of proteins with desired functional properties.
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